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OBJECTIVE:To study the effect of dihydromyricetin(DMY)on high glucose(HG)-induced glomerular mesangial cell(MCs)proliferation and fibronectin(FN)accumulation,and explore its mechanism for diabetic nephropathy glomerulosclero-sis. METHODS:Cells were divided into normal group(5.5 mmol/L glucose),HG group(30 mmol/L glucose),DMY low-concen-tration,medium-concentration,high-concentration groups (30 mmol/L glucose+22.5,45,90 μmol/L DMY). After incubating 48 h,MTT was used to detect the proliferative activity [reflected by the optical density(OD)value] of cells;molecular docking meth-od was adopted to conduct simulation analysis for DMY binding state with Smad2. Cells were divided into normal group(5.5 mmol/L glucose),HG group (30 mmol/L glucose),DMY group (30 mmol/L glucose+45 μmol/L DMY) and DMY control group (5.5 mmol/L glucose+45 μmol/L DMY). After incubating 5 h,Western blot was used to detect the expression levels of phosphorylated Smad2 (p-Smad2) and extracellular matrix protein FN. RESULTS:Results of MTT detection showed,compared with normal group,OD values in HG group were significantly increased(P<0.05);compared with HG group,OD values in DMY each con-centration group were significantly reduced(P<0.05). The Gibbs free energy(ΔG)of DMY and Smad2 protein was -5.64 kJ/mol, Ki was 73.53 μmol/L,and there were hydrogen bond donor and receptor binding in No. 465,464,461,458 amino acid residues. Results of Western blot showed,compared with normal group,expression levels of p-Smad2 and extracellular matrix protein FN in HG group were significantly increased (P<0.05);compared with HG group,expression levels of p-Smad2 and extracellular ma-trix protein FN in DMY group were significantly decreased(P<0.05). CONCLUSIONS:DMY inhibits HG-induced MCs prolifera-tion and improves diabetic nephropathy glomerulosclerosis by combining with Smad2 and inhibiting Smad2 phosphorylation to re-duce the extracellular matrix protein FN expression.
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Objective To investigate the value of 320-detector row CT angiography for evaluating diabetic dorsalis pedis arterio-sclerotic disease.Methods 18 patients with diabetes and 20 healthy volunteers underwent 320-detector row CT angiography.The obtained thin-slice images were reformatted using maximum intensity projection,volume rendering,curved planar reformation and multiplanar reconstruction to evaluate the features of dorsalis pedis artery.Results 320-detector row CT angiography could exactly show the course,morphological features of the dorsalis pedis artery and the anatomical detail of arteriosclerotic disease.Arterioscle-rostic was found in variant degree in 18 cases.Among all,6 and 7 sides were slight arteriostenosis in proximal and distal segment,re-spectively.Moreover,2 and 3 sides were medium arteriostenosis in proximal and distal segment,respectively.The most common type of plaque property were mixed plaque.Conclusion 320-detector row CT angiography is of significant value in the assessment of arte-riosclerotic disease of dorsalis pedis arteries in the patients with diabetes.
ABSTRACT
Parathyroid Hormone-related Protein( PTHrP) is a polyhormone secretory protein secreted by a variety of tissues and cells that plays fundamental roles in the growth and development of various organs, promotes migration and invasion in breast cancer, prostate cancer and non-small cell lung cancer and also plays a key role in osteolysis.With the development of scientific research, many unknown functions of the parathyroid hormone-related protein will be uncovered.
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【Objective】To observe the therapeutic effect of oral use of Neiyi Pill(composed of Radix Salviae Miltiorrhizae,Sanguis Draxonis,Rhizoma Sparganii,Rhizoma Curcumae,Semen Persicae,Radix Notoginseng,Radix Angelicae Sinensis,Ramulus Cinnamomi,Rhizoma Cyperi,Radix Achyranthis Bidentatae,etc.) combined with enteroclysis of Neiyi Enema(composed of Radix Salviae Miltiorrhizae,Sanguis Draxonis,Semen Persicae,Rhizoma Curcumae,Radix Paeoniae Rubra,Rhizoma Polygoni Cuspidati,etc.) for dysmenorrhea induced by endometriosis(EMS).【Methods】Fifty-eight patients diagnosed as endometriosis by laparoscopy were divided into 3 groups by randomized single-blind method.Group A(n=16) was treated with Neiyi Pill orally,Group B(n=24) with Neiyi Pill orally combined with enteroclysis of Neiyi Enema and Group C(n=18) with danazol orally.The 3 groups respectively received the corresponding treatment for 3 successive menstrual cycles(MC).Dysmenorrhea scoring was carried out according to the Guide for Clinical Research of New Chinese Herbal Medicine before treatment and after 1,2 and 3 menstrual cycles.The effects on symptoms of dysmenorrhea,lumbosacral discomfort and irritative symptom of rectum,signs of tenderness and nodule in posterior fornix of vagina and adnexal masses,and syndrome of blood stasis were observed.Meanwhile,the adverse reactions were also monitored.【Results】After treatment,effect on relieving dysmenorrhea in group B was superior to that in group C(P0.05).Effect on lumbosacral discomfort and syndrome of blood stasis in groups A and B was superior to that in group C(P0.05).Effect on irritative symptom of rectum,tenderness and nodule in posterior fornix of vagina and adnexal masses in group B was superior to that in group C(P0.05).【Conclusion】Oral use of Neiyi Pill combined with enteroclysis of Neiyi Enema has a better effect for endometriosis than only oral use of Neiyi Pill only or danazol,and has no obvious adverse reactions.